Graduate (Medical school) molecular biology course – 2023

Facultad de Medicina, Universidad Autónoma de San Luis Potosí.

Unit 1. From August 15 to September 07, 2023.


Semester: 3rd semester
Type: Lecture series.
Total allocated time: 18 hours
Schedule: monday through friday from 11:00 to 12:00 hrs plus extra sessions on request.
Professors: Dr. Christian A. García-Sepúlveda & Dr. Sandra E. Guerra-Palomares.


Syllabus:

1. Overview: Human Genome. (Aug 15 - 16, 2023)
Download presentation (spanish ppt format, 10.6 MB)
Flow of information, overview of the human genome, brief overview of repication, transcription and translation.
Last update: Aug 12, 2022.
Supplementary documents:
The International Human Genome Project (Hum Mol Genet . 2021 Oct 1;30(R2):R161-R163.)

2. Sturcture of the human genome. (Aug 17 - 19, 2023)
Download presentation (spanish ppt format, 11.2 MB)
Doble helix basics, genomic organization and genes, gene clusters and repeats, polymorphism.
Last update: Aug 12, 2022.
Supplementary documents:
Organizational Principles of 3D Genome Architecture (Nat Rev Genet . 2018 Dec;19(12):789-800.)

3. Chromatin and epigenetics. (Aug 20 - 21, 2023)
Download presentation (spanish ppt format,33 MB)
Nucleosome structure, heterochromatin and euchromatin, histone metilation and acetylation, DNA methylation.
Last update: Aug 12, 2022.
Supplementary documents:
The Impact of Nutrition and Environmental Epigenetics on Human Health and Disease. (Int J Mol Sci. 2018 Nov 1;19(11):3425.)

4. DNA replication. (Aug 22 and 23, 2023)
Download presentation (spanish ppt format, 57.3 MB)
Replication in eukaryotes, telomerase function, mitochondrial genome replication.
Last update: Aug 12, 2022.
Supplementary documents:
Origins of DNA replication (PLoS Genet. 2019 Sep 12;15(9):e1008320.)

4. DNA repair and diseases. (Aug 24, 2023)
Download presentation (spanish ppt format, 9.9 MB)
Replication in eukaryotes, telomerase function, mitochondrial genome replication.
Last update: Aug 12, 2022.
Supplementary documents:
Mechanisms of DNA damage, repair and mutagenesis (Environ Mol Mutagen. 2017 Jun;58(5):235-263.)

5. Gene transcription. (Aug 28 - 29, 2023)
Download presentation (spanish ppt format, 6 MB)
Regulatory elements, exons and introns, transcription factors.
Last update: Aug 12, 2022.
Supplementary documents:
Visualizing transcription: key to understanding gene expression dynamics (Curr Opin Chem Biol. 2019 Aug;51:122-129.)

8. mRNA, rRNA and tRNA. (Aug 30, 2023)
Download presentation (spanish ppt format, 3.3 MB)
mRNA synthesis and post-transcriptional modifications, tRNA synthesis and structure, rRNA structure.
Last update: Aug 12, 2022.
Supplementary documents:
Drug delivery systems for RNA therapeutics. (Nat Rev Genet. 2022 May;23(5):265-280.)

9. siRNA. (Aug 31, 2023)
Download presentation (spanish ppt format, 3.8 MB)
Interference RNA, micro RNAs and silencing RNAs.
Last update: Aug 12, 2022.
Supplementary documents:
Therapeutic siRNA: principles, challenges, and strategies (Yale J Biol Med. 2012 Jun;85(2):187-200.)

10. The Genetic Code. (Sept 01 - 02, 2023)
Download presentation (spanish ppt format, 2.8 MB)
Clasical and alternative genetic codes.
Last update: Aug 12, 2022.
Supplementary documents:
Evolution of the genetic code (Transcription. 2021 Feb;12(1):28-53.)

11. Translation. (Sept 03 - 06, 2023)
Download presentation (spanish ppt format, 2.6 MB)
The genetic code, translation events, ribosomal function.
Last update: Aug 12, 2022.
Supplementary documents:
How Macrolide Antibiotics Work. (Trends Biochem Sci. 2018 Sep;43(9):668-684.)


12. First evaluation. (Sept 07, 2023)